anti filaggrin Search Results


flg  (Novus Biologicals)
94
Novus Biologicals flg
Flg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti filaggrin  (R&D Systems)
92
R&D Systems anti filaggrin
Anti Filaggrin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti filaggrin antibody  (Santa Cruz Biotechnology)
95
Santa Cruz Biotechnology rabbit anti filaggrin antibody
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Rabbit Anti Filaggrin Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti filaggrin  (Biorbyt)
93
Biorbyt anti filaggrin
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Anti Filaggrin, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse ab against filaggrin  (Novus Biologicals)
91
Novus Biologicals mouse ab against filaggrin
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Mouse Ab Against Filaggrin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti flg  (Novus Biologicals)
92
Novus Biologicals anti flg
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Anti Flg, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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filaggrin  (Novus Biologicals)
91
Novus Biologicals filaggrin
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Filaggrin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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guinea pig  (Cusabio)
86
Cusabio guinea pig
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Guinea Pig, supplied by Cusabio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse flg antibody  (Novus Biologicals)
90
Novus Biologicals mouse flg antibody
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Mouse Flg Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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polyclonal antifilaggrin (against the 37 kd, monomer)  (Covance)
90
Covance polyclonal antifilaggrin (against the 37 kd, monomer)
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Polyclonal Antifilaggrin (Against The 37 Kd, Monomer), supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti-filaggrin  (Sanbio Inc)
90
Sanbio Inc mouse monoclonal anti-filaggrin
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Mouse Monoclonal Anti Filaggrin, supplied by Sanbio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-human/mouse filaggrin  (Covance)
90
Covance rabbit anti-human/mouse filaggrin
FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) <t>Filaggrin</t> and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.
Rabbit Anti Human/Mouse Filaggrin, supplied by Covance, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) Filaggrin and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.

Journal: Frontiers in pharmacology

Article Title: Edible Bird's Nest, an Asian Health Food Supplement, Possesses Moisturizing Effect by Regulating Expression of Filaggrin in Skin Keratinocyte.

doi: 10.3389/fphar.2021.685982

Figure Lengend Snippet: FIGURE 2 | Expressions of filaggrin and filaggrin-2 are evaluated by real- time PCR. (A) The schematic diagrams of primers flanking the exons of human filaggrin (FLG) and filaggrin-2 (FLG2) genes on chromosome 1q21 are indicated. (B) Filaggrin and filaggrin-2 mRNA levels in cultured differentiated (upper panel; 1.8 mM Ca2+) and undifferentiated (lower panel; low Ca2+) keratinocytes after 24-h treatments of EBN extract and digest, in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. Values are expressed as the percentage of increase in relative to normalized basal expression set at 0, in mean ± SEM, n 4. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.

Article Snippet: Primary antibodies used: mouse and rabbit anti-filaggrin antibody at 1:100 (Santa Cruz Biotechnology, Dallas, United States, catalog number: sc-66192 and sc-30229), rabbit anti-filaggrin-2 antibody at 1:1,000 (Bethyl Laboratories, Montgomery, United States, catalog number: A305-861A-M), rabbit anti-p38 MAPK antibody at 1:1,000 (Cell Signaling Technology, Danvers, United States, catalog number: 8690S), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) antibody at 1: 1,000 (Cell Signaling Technology, catalog number: 4511S), rabbit anti-GATA3 antibody at 1:1,000 (Cell Signaling Technology, catalog number: 5852S), rabbit anti-phospho-GATA3 (Ser308) antibody at 1:1,000 (Thermo Fisher Scientific, catalog number: MA5-32144), rabbit anti-PPARα + PPARβ antibody at 1:2,000 (Abcam Ltd., Cambridge, United Kingdom, catalog number: ab1788865), rabbit anti-PPARγ antibody at 1:1,000 (Cell Signaling Technology, catalog number: 2435S), rabbit anti-CASP14 antibody at 1:1,000 (Cell Signaling Technology, catalog number: 8519S), and mouse antiα-tubulin antibody at 1:10,000 (Sigma-Aldrich, catalog number: 3873S).

Techniques: Real-time Polymerase Chain Reaction, Cell Culture, Positive Control, Expressing, Control

FIGURE 3 | EBN induces the activity of pFLG2-eGFP and filaggrin expression. (A) The fluorescence images of pFLG2-eGFP transfected keratinocytes after 24-h treatments of CaCl2 (0.16 mM, a positive control), EBN extract (100 μg/mL), and EBN digest (100 μg/mL) are shown, n 3. Bar 100 μm. The pFLG2-eGFP plasmid was composed of FLG2 promoter tagged with EGFP reporter. (B) The quantifications from (A) are expressed as the percentage of increase in fluorescence intensity measured under excitation/emission wavelength 488/509 nm to normalized basal activity set at 0 in mean ± SEM, n 6. (C) Cytosolic expression of FLG protein in HaCaT keratinocytes after 24-h treatments of EBN extract (100 μg/mL) and EBN digest (100 μg/mL) are shown, in representative confocal images. CaCl2 at 0.16 mM was adopted as a positive control. n 3. Bar 20 μm. (D) The quantifications from (C) are expressed as the percentage increase in filaggrin fluorescence intensity to normalized basal activity set at 0 in mean ± SEM, n 3. The filaggrin fluorescence intensity of each cell was normalized to total cell area for quantification. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.

Journal: Frontiers in pharmacology

Article Title: Edible Bird's Nest, an Asian Health Food Supplement, Possesses Moisturizing Effect by Regulating Expression of Filaggrin in Skin Keratinocyte.

doi: 10.3389/fphar.2021.685982

Figure Lengend Snippet: FIGURE 3 | EBN induces the activity of pFLG2-eGFP and filaggrin expression. (A) The fluorescence images of pFLG2-eGFP transfected keratinocytes after 24-h treatments of CaCl2 (0.16 mM, a positive control), EBN extract (100 μg/mL), and EBN digest (100 μg/mL) are shown, n 3. Bar 100 μm. The pFLG2-eGFP plasmid was composed of FLG2 promoter tagged with EGFP reporter. (B) The quantifications from (A) are expressed as the percentage of increase in fluorescence intensity measured under excitation/emission wavelength 488/509 nm to normalized basal activity set at 0 in mean ± SEM, n 6. (C) Cytosolic expression of FLG protein in HaCaT keratinocytes after 24-h treatments of EBN extract (100 μg/mL) and EBN digest (100 μg/mL) are shown, in representative confocal images. CaCl2 at 0.16 mM was adopted as a positive control. n 3. Bar 20 μm. (D) The quantifications from (C) are expressed as the percentage increase in filaggrin fluorescence intensity to normalized basal activity set at 0 in mean ± SEM, n 3. The filaggrin fluorescence intensity of each cell was normalized to total cell area for quantification. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.

Article Snippet: Primary antibodies used: mouse and rabbit anti-filaggrin antibody at 1:100 (Santa Cruz Biotechnology, Dallas, United States, catalog number: sc-66192 and sc-30229), rabbit anti-filaggrin-2 antibody at 1:1,000 (Bethyl Laboratories, Montgomery, United States, catalog number: A305-861A-M), rabbit anti-p38 MAPK antibody at 1:1,000 (Cell Signaling Technology, Danvers, United States, catalog number: 8690S), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) antibody at 1: 1,000 (Cell Signaling Technology, catalog number: 4511S), rabbit anti-GATA3 antibody at 1:1,000 (Cell Signaling Technology, catalog number: 5852S), rabbit anti-phospho-GATA3 (Ser308) antibody at 1:1,000 (Thermo Fisher Scientific, catalog number: MA5-32144), rabbit anti-PPARα + PPARβ antibody at 1:2,000 (Abcam Ltd., Cambridge, United Kingdom, catalog number: ab1788865), rabbit anti-PPARγ antibody at 1:1,000 (Cell Signaling Technology, catalog number: 2435S), rabbit anti-CASP14 antibody at 1:1,000 (Cell Signaling Technology, catalog number: 8519S), and mouse antiα-tubulin antibody at 1:10,000 (Sigma-Aldrich, catalog number: 3873S).

Techniques: Activity Assay, Expressing, Transfection, Positive Control, Plasmid Preparation, Control

FIGURE 4 | EBN induces the protein levels of filaggrin and filaggrin-2. (A) The protein levels of filaggrin (FLG) and filaggrin-2 (FLG2) in cultured differentiated keratinocytes, after 24-h treatments of EBN extract and digest, were determined in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. (B) The total filaggrin (summation of the isomeric intermediates) and (C) filaggrin-2 protein levels, relative to α-tubulin protein, are expressed as the percentage increase to normalized basal expression set at 0 in mean ± SEM, n 3. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.

Journal: Frontiers in pharmacology

Article Title: Edible Bird's Nest, an Asian Health Food Supplement, Possesses Moisturizing Effect by Regulating Expression of Filaggrin in Skin Keratinocyte.

doi: 10.3389/fphar.2021.685982

Figure Lengend Snippet: FIGURE 4 | EBN induces the protein levels of filaggrin and filaggrin-2. (A) The protein levels of filaggrin (FLG) and filaggrin-2 (FLG2) in cultured differentiated keratinocytes, after 24-h treatments of EBN extract and digest, were determined in doses of 1, 10, 100 μg/mL, as indicated. CaCl2 at 0.16 mM was adopted as a positive control. (B) The total filaggrin (summation of the isomeric intermediates) and (C) filaggrin-2 protein levels, relative to α-tubulin protein, are expressed as the percentage increase to normalized basal expression set at 0 in mean ± SEM, n 3. Statistically significant results are marked with * p < 0.05, ** p < 0.01, and *** p < 0.001 against the control.

Article Snippet: Primary antibodies used: mouse and rabbit anti-filaggrin antibody at 1:100 (Santa Cruz Biotechnology, Dallas, United States, catalog number: sc-66192 and sc-30229), rabbit anti-filaggrin-2 antibody at 1:1,000 (Bethyl Laboratories, Montgomery, United States, catalog number: A305-861A-M), rabbit anti-p38 MAPK antibody at 1:1,000 (Cell Signaling Technology, Danvers, United States, catalog number: 8690S), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) antibody at 1: 1,000 (Cell Signaling Technology, catalog number: 4511S), rabbit anti-GATA3 antibody at 1:1,000 (Cell Signaling Technology, catalog number: 5852S), rabbit anti-phospho-GATA3 (Ser308) antibody at 1:1,000 (Thermo Fisher Scientific, catalog number: MA5-32144), rabbit anti-PPARα + PPARβ antibody at 1:2,000 (Abcam Ltd., Cambridge, United Kingdom, catalog number: ab1788865), rabbit anti-PPARγ antibody at 1:1,000 (Cell Signaling Technology, catalog number: 2435S), rabbit anti-CASP14 antibody at 1:1,000 (Cell Signaling Technology, catalog number: 8519S), and mouse antiα-tubulin antibody at 1:10,000 (Sigma-Aldrich, catalog number: 3873S).

Techniques: Cell Culture, Positive Control, Expressing, Control

FIGURE 10 | EBN induces filaggrin in ex vivo mouse skin. (A) The expression of filaggrin (FLG) protein in 2-month-old mouse dorsal skin ex vivo after 24-h treatments of EBN extract (100 μg/mL) and EBN digest (100 μg/mL) were assessed by immunofluorescent staining. CaCl2 at 0.16 mM was adopted as a positive control, n 3. Bar 20 μm. Yellow dash lines indicate the fluorescent area of filaggrin. (B) The epidermal thickness of ex vivo mouse dorsal skin after 24-h treatments of CaCl2 (0.16 mM), EBN extract (100 μg/mL) and EBN digest (100 μg/mL) were assessed by H and E staining. Black dash lines include epidermis layer (right panel). The quantification of five randomly selected distance points were assessed for the epidermal thickness (left panel). n 3. Bar 100 μm.

Journal: Frontiers in pharmacology

Article Title: Edible Bird's Nest, an Asian Health Food Supplement, Possesses Moisturizing Effect by Regulating Expression of Filaggrin in Skin Keratinocyte.

doi: 10.3389/fphar.2021.685982

Figure Lengend Snippet: FIGURE 10 | EBN induces filaggrin in ex vivo mouse skin. (A) The expression of filaggrin (FLG) protein in 2-month-old mouse dorsal skin ex vivo after 24-h treatments of EBN extract (100 μg/mL) and EBN digest (100 μg/mL) were assessed by immunofluorescent staining. CaCl2 at 0.16 mM was adopted as a positive control, n 3. Bar 20 μm. Yellow dash lines indicate the fluorescent area of filaggrin. (B) The epidermal thickness of ex vivo mouse dorsal skin after 24-h treatments of CaCl2 (0.16 mM), EBN extract (100 μg/mL) and EBN digest (100 μg/mL) were assessed by H and E staining. Black dash lines include epidermis layer (right panel). The quantification of five randomly selected distance points were assessed for the epidermal thickness (left panel). n 3. Bar 100 μm.

Article Snippet: Primary antibodies used: mouse and rabbit anti-filaggrin antibody at 1:100 (Santa Cruz Biotechnology, Dallas, United States, catalog number: sc-66192 and sc-30229), rabbit anti-filaggrin-2 antibody at 1:1,000 (Bethyl Laboratories, Montgomery, United States, catalog number: A305-861A-M), rabbit anti-p38 MAPK antibody at 1:1,000 (Cell Signaling Technology, Danvers, United States, catalog number: 8690S), rabbit anti-phospho-p38 MAPK (Thr180/Tyr182) antibody at 1: 1,000 (Cell Signaling Technology, catalog number: 4511S), rabbit anti-GATA3 antibody at 1:1,000 (Cell Signaling Technology, catalog number: 5852S), rabbit anti-phospho-GATA3 (Ser308) antibody at 1:1,000 (Thermo Fisher Scientific, catalog number: MA5-32144), rabbit anti-PPARα + PPARβ antibody at 1:2,000 (Abcam Ltd., Cambridge, United Kingdom, catalog number: ab1788865), rabbit anti-PPARγ antibody at 1:1,000 (Cell Signaling Technology, catalog number: 2435S), rabbit anti-CASP14 antibody at 1:1,000 (Cell Signaling Technology, catalog number: 8519S), and mouse antiα-tubulin antibody at 1:10,000 (Sigma-Aldrich, catalog number: 3873S).

Techniques: Ex Vivo, Expressing, Staining, Positive Control